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J Biochem Tech
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| Volume 2, Issue 1, Dec 2009, Page No. 126-132 |
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| Copyrights © 2008 , Sevas Educational Society, All rights Reserved | ||||||||||
| Xylitol production from lactose by biotransformation |  |
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Tomoyuki Toyoda and Kazuhisa Ohtaguchi |
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| Department of Chemical Engineering, Tokyo Institute of Technology, Tokyo 2-12-1 Ookayama, Meguro-Ku, Tokyo 152- 8552, Japan | ||||||||||
| Received: 28 November 2009 / Received in revised form: 3 February 2010, Accepted: 13 February 2010, Published online: 11 March 2010 | ||||||||||
| Abstract | ||||||||||
| Xylitol production from lactose was investigated by a three step biotransformations series that were: (1) breakdown of lactose to Darabitol by Kluyveromyces lactis NBRC 1903, (2) oxidation of Darabitol to D-xylulose by Gluconobacter oxydans NBRC 3172, and (3) reduction of D-xylulose to xylitol by Candida shehatae IAM 12953 in the presence of a high concentration ethanol or by cell free sorbitol dehydrogenase. Overall production rate and overall fractional yield of xylitol from lactose were 1.69 mmol L-1 h-1 and 0.141, respectively, when sorbitol dehydrogenase was utilized in the third reaction. The results of xylitol production from lactose were compared with the processes of xylitol production from D-glucose. |
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| Keywords: Xylitol, Lactose, Kluyveromyces lactis, Biotransformation | ||||||||||
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