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J Biochem Tech
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| Volume 2, Issue 2, Apr 2010, Page No. 154-157 |
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| Copyrights © 2008 , Sevas Educational Society, All rights Reserved | ||||||||||
| Statistical
optimization of endo-polygalacturonase production by overproducing mutants of Aspergillus niger in solid-state fermentation |
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R R Siva Kiran, Rajesh Konduri, G H Rao , G M Madhu |
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*Center for
Biotechnology, Department of Chemical Engineering, |
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| Received: 18 November 2009 / Received in revised form: 18 December 2009, Accepted: 31 December 2009, Published online: 10 January 2010 | ||||||||||
| Abstract | ||||||||||
| Statistically based experimental
designs were applied for endopolygalacturonase production by mutated strains
of Aspergillus niger using apple pomace waste in solid-state fermentation (SSF). The Plackett-Burman design was used to search for the main factors. Urea was found to be the most significant factor (p<0.05) among these variables. To study the mutual interactions between variables and find the optimum medium, Doehlert design was performed to investigate the effect of the medium components, apple pomace, glucose and urea. Using the optimized medium, the maximal activity of endo-polygalacturonase production was found to be 4.26672 U. The Plackett-Burman design coupled with Doehlert design were proved to be the potent tools in optimizing medium composition for producing endo-polygalacturonase. |
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| Keywords:
Doehlert design, Plackett-Burman design, solidstate fermentation |
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