Secreted protease and activity of an endoproteinase for preliminary characterization of clinical Candida isolates

J Biochem Tech

Volume 2, Issue 2, Apr 2010, Page No. 168-169

Secreted protease and activity of an endoproteinase for preliminary characterization of clinical Candida isolates

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*Department of Plant Biology and Biotechnology (formerly Botany), University of Benin, PMB 1154, Benin City, Nigeria

Received: 23 September 2009 / Received in revised form: 29 September 2009, Accepted: 15 July 2010, Published online: 25 July 2010

Abstract

Nine clinical Candida strains (Candida dublinienses (one),
Candida albicans (six), and C. tropicalis (two)) were characterized
by production of secreted protease, activity of endoproteinase A
and with the HiCandida identification kit. Protease secretion was in
the range of 1.3-1.8 cm while PrA activity was between 0.19 and
0.44 μg/min/mg protein. Results are indicative of the possible use of
endoproteinase activity and SAP production for characterizing
Candida strains.

Keywords:Biochemical activity, Candida, characterization,
endoproteinase, secreted protease.

*Tel: 007 098805057

J Biochem Tech

Volume 2, Issue 2, Apr 2010, Page No. 168-169




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